Amine Dehydrogenase (AmDH)
★ motuhake tïpako High.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohenga he tïpako, he wairewa buffer, he enzyme, he coenzyme me te pünaha whakaora hou (hei tauira, te glucose me te glucose dehydrogenase).
➢ Me whakauru whakamutunga te AmDH ki roto i te punaha tauhohenga, i muri i te whakatikanga o te pH me te pāmahana ki te ahua o te tauhohenga.
Tauira 1(Ko te whakahiatotanga o nga amine chiral e rite ana ma te whakaurunga whakaheke o nga alkyl aryl ketones)(1):
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. Kong W, Liu Y, Huang C, et al.Angewandte Chemie International Edition, 2022: e202202264.