Cyclohexanone monooxygenase (CHMO)
Whakaahuatanga Poto:
Mo te Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): he Baeyer Villiger monooxygenase (BVMO) me te cyclohexanone hei taputapu, ka taea te oxidize cyclohexanone ki te cyclohexanol ma te whakamahi i te hāora.
He momo whākōkī e whakawhirinaki ana ki te flavin.Ka whakahekehia te flavin kua waikura ki te flavin na roto i te huringa o te whakaiti i te coenzyme II (NADPH) mo te waikura e whai ake nei.No reira, ko te NADPH te cofactor e tika ana i roto i te huringa flavin.Kotahi noa te ahua o te hua enzyme CHMO (Tau hei ES-CHMO101) i hangaia e SyncoZymes, ka taea te whakamahi ki te oxidize cyclohexanone ki cyclohexanone.
Momo tauhohenga catalytic:
Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-Mēra:lchen@syncozymes.com
| Nga Enzymes | Waehere Hua | Whakatakotoranga |
| Kete Tirohanga (SynKit) | ES-CHMO-101 | he huinga o 1 Ketoreductases, 1 mg ia 1 tūemi * 1mg / tūemi |
★ motuhake tïpako High.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohenga he tïpako, he wairewa buffer, he enzyme, he coenzyme me te pünaha whakaora hou (hei tauira, te glucose me te glucose dehydrogenase).
➢ Me whakauru te CHMO whakamutunga ki te punaha tauhohenga, i muri i te whakatikanga o te pH me te pāmahana ki te ahua o te tauhohenga.
Tauira 1(Te whakahiato o te cyclohexanol mai i te cyclohexanol)(1):
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
