Hydroaminase (HAM)
Nga Enzymes | Waehere Hua | Whakatakotoranga |
Te paura Enzyme | ES-HAM-101~ ES-HAM-102 | he huinga o te 2 Nuclosidephosphoryalse, 50 mg ia 2 mea * 50mg / taonga, etahi atu rahinga ranei |
Ka taea te whakamahi i te punaha tauhohenga e whai ake nei hei tohutoro:
1-10 mg/ml: Raupapa
10 mg/ml: Enzyme
10% (v/v): Whakarewa pararopi (1)
10% (v/v): Hamoni
Whakaritea te pH ki te 9.0 i mua i te taapiri i te whākōkī, ka mutu te tāpiri i te whākōkī.Whakanuia nga tauhohenga ki te wiriwiri mahana tonu (hei tauira, 30oC, 150rpm) mo te 24~48 haora.Whakanuia ia ranunga ki te whakaheke i te pūmua, ka taea te whakamahi i te supernatant mo te TLC, HPLC, GC ranei tātaritanga.
Tuhipoka (1): Ko te whakarewa pararopi (penei i te DMSO, methanol, acetonitrile, IPA, me etahi atu) he mea whiriwhiri, whakamahia mo nga taputapu korekore.
Tuhia to korero ki konei ka tukuna mai ki a matou