Karekau CALB
Ko te CALB ka kore e nekehia e te urunga tinana ki runga i te kapia tino waipoka, he polymer macroporous, styrene/methacrylate.He pai te CALB Immobilized mo nga tono i roto i nga whakarewa pararopi me nga punaha whakarewa-kore, ka taea te hangarua me te whakamahi ano mo nga wa maha i roto i nga ahuatanga pai.
Waehere Hua: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★He teitei ake te ngohe, teitei ake te whiriwhiringa o te chiral me te pumau.
★He pai ake te mahi i roto i nga waahanga kore-wai.
★Me ngawari te tango mai i te punaha tauhohenga, tere te whakamutu i nga tauhohenga, me te karo i te toenga pūmua i roto i te hua.
★Ka taea te hangarua me te whakamahi ano hei whakaiti i te utu.
| Mahi | ≥10000PLU/g |
| awhe pH mo te tauhohenga | 5-9 |
| Te awhe pāmahana mo te tauhohenga | 10-60 ℃ |
| Te ahua | CALB-IMMO100-A: Kowhai marama ki te parauri totoka CALB-IMMO100-B: Ma ki te marama parauri totoka |
| Te rahi o te matūriki | 300-500μm |
| Ka ngaro i te whakamaroke i te 105 ℃ | 0.5%-3.0% |
| Resin mo te immobilization | Macroporous, styrene/methacrylate polymer |
| Te whakarewa tauhohenga | Te wai, te whakarewa pararopi, me etahi atu, me te kore whakarewa ranei.Mo te tauhohenga o etahi whakarewa pararopi, ka taea te tapiri 3% te wai hei whakapai ake i te paanga tauhohenga |
| Te rahi o te matūriki | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Te whakamaramatanga o te waeine: 1 te waeine e rite ana ki te whakahiatotanga o te 1μmol mo ia meneti propyl laurate mai i te waikawa lauric me te 1-propanol i te 60 ℃.Ko te CALB-IMMP100-A me te CALB-IMMO100-B o runga ake nei e rite ana ki nga kaikawe korekore he rereke te rahi o te matūriki.
1. Momo reactor
E hāngai ana te whākōkī immobilized ki te reactor puranga kettle me te reactor rere haere tonu o te moenga.Me tohu kia kore e pakaru na te kaha o waho i te wa e whangai ana, e whakaki ana ranei.
2. Tauhohenga pH, pāmahana me te whakarewa
Ko te whākōkī karekau me tāpiri ki muri, i muri i te tāpiritanga me te rewa etahi atu rauemi, me te whakatika pH.
Mena ko te kai o te tïpako, ko te hanga hua ranei ka huri te pH i te wa o te tauhohenga, me tapiri he parepare ki te punaha tauhohenga, me aroturuki ranei te pH i te wa o te tauhohenga.
I roto i te awhe whakamaarama o te CALB (i raro iho i te 60 ℃), ka piki te tere o te hurihanga me te piki o te pāmahana.I roto i te whakamahi mahi, me whiriwhiri te pāmahana tauhohenga kia rite ki te pumau o te tïpako hua ranei.
Ko te tikanga, he pai te tauhohenga hauwai hauwai i roto i te punaha wahanga wai, i te mea he pai te tauhohenga whakahiato hākawa ki te punaha wahanga waro.Ko te whakarewa pararopi ko te waiwaro, te tetrahydrofuran, te n-hexane, te n-heptane me te toluene, he whakarewa whakauru tika ranei.Mo te tauhohenga o etahi whakarewa pararopi, ka taea te tapiri 3% te wai hei whakapai ake i te paanga tauhohenga.
3. Te whakamahi me te oranga o te CALB
I raro i te ahuatanga tauhohenga e tika ana, ka taea te whakaora me te whakamahi ano i te CALB, ka rereke nga wa tono motuhake me nga kaupapa rereke.
Ki te kore e whakamahia tonutia te CALB kua ora ake, me penapena i muri i te whakaoranga, me horoi, ka whakamarokehia me te hiri ki te 2-8 ℃.
Whai muri i te maha o nga rauna o te whakamahi ano, mena ka iti te kaha o te tauhohenga, ka taea te taapiri i te CALB me te whakamahi tonu.Mena ka tino whakahekehia te kaha o te urupare, me whakakapi.
Tauira 1(Aminolysis)(1):
Tauira 2(Aminolysis)(2):
Tauira 3(Whakawhiti whakakai polyester synthesis)(3):
Tauira 4(Transesterification, regioselective of hydroxyl group)(4):
Tauira 5(Transesterification, taumira kinetic o nga waipiro racemic)(5):
Tauira 6(Te whakakorikori, te taumira hiko o te waikawa carboxylic)(6):
Tauira 7(Esterolysis, taumira hiko)(7):
Tauira 8(Hydrolysis of amides)(8):
Tauira 9(Acylation of amines)(9):
Tauira 10(Aza-Michael tauhohenga taapiri)(10):
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