CALB kua kore e nekeneke
Ka whakapūmautia te CALB mā te mimiti ā-tinana ki runga i te kapia wai-kore tino pai, he pūmua styrene/methacrylate macroporous. He pai te CALB whakapūmau mō ngā tono i roto i ngā whakarewa waro me ngā pūnaha kore-whakarewa, ā, ka taea te hangarua me te whakamahi anō mō ngā wā maha i raro i ngā āhuatanga e tika ana.
Waehere Hua: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★He nui ake te ngohe, he nui ake te whiriwhiringa chiral me te pumau ake.
★He pai ake te mahi i roto i ngā wāhanga kore-wai.
★Tangohia ngāwari mai i te pūnaha tauhohenga, whakamutua wawetia ngā tauhohenga, ā, karohia hoki te toenga pūmua i roto i te hua.
★Ka taea te hangarua me te whakamahi anō hei whakaiti i te utu.
| Mahi | ≥10000PLU/g |
| Awhe pH mō te tauhohenga | 5-9 |
| Awhe pāmahana mō te tauhohenga | 10-60℃ |
| Āhua | CALB-IMMO100-A: Kōwhai mārama ki te parauri totoka CALB-IMMO100-B: He mā ki te parauri mārama te totoka |
| Rahi o te matūriki | 300-500μm |
| Ngaronga i te whakamaroke i te 105℃ | 0.5%-3.0% |
| Resin mō te ārai i te nekenekehanga | Pūmua styrene/methacrylate, he nui ngā pūngao |
| Te whakarewa tauhohenga | Wai, whakarewa waro, me ētahi atu, kāore rānei he whakarewa. Mō te tauhohenga i roto i ētahi whakarewa waro, ka taea te tāpiri i te 3% wai hei whakapai ake i te pānga tauhohenga. |
| Rahi o te matūriki | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Whakamāramatanga o te waeine: 1 waeine e rite ana ki te hanganga o te 1μmol ia meneti propyl laurate mai i te waikawa lauric me te 1-propanol i te 60℃. Ko te CALB-IMMP100-A me te CALB-IMMO100-B i runga ake nei e rite ana ki ngā kaikawe kua kore e nekeneke me ngā rahi matūriki rerekē.
1. Momo tauhohenga
Ka taea te whakamahi i te whākōkī kua whakapūmautia mō te tauhohenga puranga ipu me te tauhohenga rere tonu o te moenga pumau. Me mōhio kia kaua e kuruhia e te kaha o waho i te wā e whāngai ana, e whakakī ana rānei.
2. Te pH tauhohenga, te pāmahana me te whakarewa
Ko te whākōkī kua kore e nekeneke te mea whakamutunga, whai muri i te tāpiri i ētahi atu rauemi, me te whakarewa, me te whakatikatika i te pH.
Mena ka huri te pH i te wā o te tauhohenga i te kai o te papa, i te hanganga rānei o te hua, me tāpiri he pākawa waro ki te pūnaha tauhohenga, me aroturuki rānei te pH, me whakatikatika hoki i te wā o te tauhohenga.
I roto i te awhe ātete pāmahana o CALB (i raro iho i te 60 ℃), ka piki ake te tere tahuritanga me te pikinga o te pāmahana. I roto i te whakamahinga mahi, me whiriwhiri te pāmahana tauhohenga kia rite ki te pumau o te papa, o te hua rānei.
I te nuinga o te wā, he pai te tauhohenga waikura ester i roto i te pūnaha āhua wai, engari he pai te tauhohenga hanga ester i roto i te pūnaha āhua waro. Ko te whakarewa waro ka taea te ethanol, te tetrahydrofuran, te n-hexane, te n-heptane me te toluene, he whakarewa whakauru rānei e tika ana. Mō te tauhohenga i roto i ētahi whakarewa waro, ka taea te tāpiri i te 3% wai hei whakapai ake i te pānga tauhohenga.
3. Te whakamahinga anō me te roanga o te ora o te CALB
I raro i te āhua tauhohenga tika, ka taea te whakaora me te whakamahi anō i te CALB, ā, ka rerekē ngā wā tono motuhake i runga i ngā kaupapa rerekē.
Mena kāore te CALB i whakahokia mai e whakamahia tonutia ana, ā, me penapena i muri i te whakahokinga mai, me horoi, me whakamaroke, ā, me hiri ki te 2-8 ℃.
I muri i ētahi whakamahinga anō, ki te iti haere te whai huatanga o te tauhohenga, ka taea te tāpiri i te CALB me te whakamahi tonu. Ki te tino heke te whai huatanga o te tauhohenga, me whakakapi.
Tauira 1 (Aminolysis)(1):
Tauira 2 (Aminolysis)(2):
Tauira 3 (Te hanganga polyester whakatuwheratanga mowhiti)(3):
Tauira 4 (Te whakawhiti i te esterification, te whiriwhiringa rohe o te rōpū hydroxyl)(4):
Tauira 5 (Te Whakapūtanga Whakawhiti, te whakataunga nekeneke o ngā waipiro racemic)(5):
Tauira 6 (Te whakapūtanga, te taumira nekeneke o te waikawa carboxylic)(6):
Tauira 7 (Esterolysis, taumira nekeneke)(7):
Tauira 8 (Te waikura o ngā amides)(8):
Tauira 9 (Te whakapūtanga o ngā amine)(9):
Tauira 10 (tauhohenga tāpiritanga Aza-Michael)(10):
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5. Kamble MP, Shinde SD, Yadav G DJ Mol. Catal. B: Enzym, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. Process Biochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, e tal. J. Mol. Catal. B: Enzym, 2016, 130: 58-69.
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9. Joubioux FL, Henda YB, Bridiau N, e tal. J. Mol. Catal. B: Enzym, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, e tal. Tetrahedron Lett, 2010, 51: 4455-4458.
