Ketoreductase (KRED)

Whakaahuatanga Poto:

Mo te Ketoreductase

Enzymes: He whakakōkī koiora macromolecular, he pūmua te nuinga o ngā whākōkī.

Ketoreductase: Ka karangahia ano ko "Carbonyl reductase", he whaakiki e whakakorikori ana i te tauhohenga redox whakamuri i waenga i nga aldehydes / ketones me nga waipiro, me te hiahia i nga coenzymes hei kaiwhakawhiti H penei i te NADH, NADPH ranei.E toru nga waahanga matua o te KRED: Dehydrogenase / Reductases (SDRs), Dehydrogenase / Reductases Waehenga (MDRs), me Aldo-KetoReductases (AKRs).He rite nga mahi catalytic o te toru, engari he rereke te hanga me nga ahuatanga.

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Ī-Mēra:lchen@syncozymes.com

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Mo te Ketoreductase:

KRED mai i SyncoZymes: E 191 nga momo o nga hua enzyme KRED (Tau hei ES-KRED-101~ES-KRED-291) i hangaia e SyncoZymes.Ka taea te whakamahi nui a SZ-KRED i roto i te whakahekenga-a-rohe me te whiriwhiringa hiko o te aldehydes, te beta-ketone esters, te α-ketone esters me te ketones.

Momo tauhohenga catalytic:

Mōhiohio Hua:

Nga Enzymes	Waehere Hua	Whakatakotoranga
Te paura Enzyme	Te paura Enzyme	he huinga 187 Ketoreductases, 50 mg ia 187 mea * 50mg / mea, etahi atu rahinga ranei
Kete Tirohanga (SynKit)	ES-KRED-18100	he huinga 181 Ketoreductases, 1 mg ia 181 mea * 1mg / mea

Painga:

★ motuhake tïpako teitei.
★ He kaha te whiriwhiringa o te chiral.
★ te kaha o te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.

Nga tohutohu mo te whakamahi:

➢ Me mahi te tirotiro i te haukini mo nga taputapu motuhake na te mea he motuhake te taputapu, me te tiki i tetahi whaariki e whakakorikori ana i te tïpako i whäia me te whai hua whakatiki pai rawa atu.
➢ Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi me te kukū teitei.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohenga he tïpako, he wairewa buffer, ngongo (NAD(H), NADP(H) ranei), te punaha whakaora i te coenzyme (hei tauira, te glucose me te glucose dehydrogenase).
➢ Me whakauru te KRED whakamutunga ki roto i te punaha tauhohenga, i muri i te whakatikanga o te pH me te pāmahana ki te ahuatanga tauhohenga.
➢ Ko nga momo KRED katoa he rerekee nga tikanga tauhohenga tino pai, no reira me ako ake ia takitahi.