Lactate Dehydrogenase (LDH)
Nga Enzymes | Waehere Hua | Whakatakotoranga |
Te paura Enzyme | ES-LDH-101 | he huinga o 1 Monoamine Oxidase, 50 mg ia 1 mea * 50mg / mea, etahi atu rahinga ranei |
Ka taea te whakamahi i te punaha tauhohenga e whai ake nei hei tohutoro:
1-10 mg/ml: Raupapa
10 mg/ml: Enzyme
10% (v/v): Whakarewa pararopi (1)
100 mM: Pūreihana Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Hangaia te haukinia
10 mg/ml: Whakahōputu dehydrogenase (FDH)
Whakanuia nga tauhohenga ki te wiriwiri mahana tonu (hei tauira, 30oC, 150rpm) mo te 24~48 haora.Whakanuia ia ranunga ki te whakaheke i te pūmua, ka taea te whakamahi i te supernatant mo te tātari TLC, HPLC, GC ranei.
Tuhipoka (1): Ko te whakarewa pararopi (penei i te DMSO, methanol, acetonitrile, IPA, me etahi atu) he mea whiriwhiri, whakamahia mo nga taputapu korekore.
Tuhia to korero ki konei ka tukuna mai ki a matou