Leucine Dehydrogenase (LeuDH)
Whakaahuatanga Poto:
Mo te Leucine dehydrogenase
He 1 momo hua enzyme LeuDH (Tau hei ES-LeuDH-101) i hangaia e SyncoZymes.Ka taea e LeuDH te whakakorikori i te haukini whakaheke o te waikawa 4-methylbutanoic (tote) ki te L-leucine, te whakapouritanga ranei o te leucine ki te waikawa 4-methylbutanoic.Ka taea hoki e ES-LeuDH te whakakorikori i te whakaurunga whakaheke o nga waikawa α-keto me te hanga rite ki te whakarite i nga hua o te waikawa amino e rite ana.I roto i te tukanga o te catalysis, e tika ana te NADH hei kawe hauwai.
Leucine dehydrogenasecatalytic momo tauhohenga
Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-Mēra:lchen@syncozymes.com
| Nga Enzymes | Waehere Hua | Whakatakotoranga |
| Te paura Enzyme | ES-LeuDH-101 | he huinga o 1 Monoamine Oxidase, 50 mg ia 1 mea * 50mg / mea, etahi atu rahinga ranei |
Ka taea te whakamahi i te punaha tauhohenga e whai ake nei hei tohutoro:
1-10 mg/ml: Raupapa
10 mg/ml: Enzyme
10% (v/v): Whakarewa pararopi (1)
100 mM: Pūreihana Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Hangaia te haukinia
10 mg/ml: Whakahōputu dehydrogenase (FDH)
Whakanuia nga tauhohenga ki te wiriwiri mahana tonu (hei tauira, 30oC, 150rpm) mo te 24~48 haora.Whakanuia ia ranunga ki te whakaheke i te pūmua, ka taea te whakamahi i te supernatant mo te tātari TLC, HPLC, GC ranei.
Tuhipoka (1): Ko te whakarewa pararopi (penei i te DMSO, methanol, acetonitrile, IPA, me etahi atu) he mea whiriwhiri, whakamahia mo nga taputapu korekore.
