Te Leucine Dehydrogenase (LeuDH)
Whakaahuatanga Poto:
Mō te Leucine dehydrogenase
Kotahi te momo hua whākōkī LeuDH (ko te nama ko ES-LeuDH-101) i whakawhanakehia e SyncoZymes. Ka taea e LeuDH te whakakorikori i te whakakāhuinga whakaiti o te waikawa 4-methylbutanoic (tote) ki te L-leucine, te whakakōkiritanga rānei o te leucine ki te waikawa 4-methylbutanoic. Ka taea hoki e ES-LeuDH te whakakorikori i te whakakōkiritanga whakaiti o ngā waikawa α-keto me te hanganga rite hei whakarite i ngā matū waikawa amino e rite ana. I roto i te tukanga whakakorikori, he mea nui te NADH hei kaikawe hauwai.
Momo tauhohenga catalytic Leucine dehydrogenase
Waea Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-mēra:lchen@syncozymes.com
| Ngā whākōkī | Waehere Hua | Whakatakotoranga |
| Paura whākōkī | ES-LeuDH-101 | he huinga o te 1 Monoamine Oxidase, 50 mg ia 1 tūemi * 50mg ia tūemi, tētahi atu rahinga rānei |
Hei tauira, ka taea te whakamahi i te pūnaha tauhohenga e whai ake nei:
1-10 mg/ml: Papatūānuku
10 mg/ml: Whākōkī
10% (v/v): Wairewa whakakoi waro (1)
100 mM: Pūnaha ārai phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Pūmua haukini
10 mg/ml: Te whakapūngao whakapūngao (FDH)
Whakatōkia ngā tauhohenga ki tētahi rūrū pāmahana pumau (hei tauira, 30°C, 150rpm) mō ngā hāora 24~48. Whakakotahitia ia ranunga ki te pūmua, ā, ka taea te whakamahi i te waikura mō te tātaritanga TLC, HPLC, GC rānei.
Tuhipoka (1): He kōwhiringa te whakarewa rauropi (pērā i te DMSO, te methanol, te acetonitrile, te IPA, me ētahi atu), e whakamahia ana mō ngā matū kore-rewa.
