Nucloside phosphoryalse (NP)

Whakaahuatanga Poto:

Mō te phosphoryalse nucleoside

E toru ngā momo hua whākōkī NP (Tau ko ES-NP-101~ ES-NP-103) i whakawhanakehia e SyncoZymes. Ko ES-NP-101 he purine nucleoside phosphorylase, ko ES-NP-102 me ES-NP-103 he pyrimidine nucleoside phosphorylase. Ka taea e te nucleoside phosphorylase te wawahi i ngā nucleoside ki ngā turanga me te pentose phosphate. Ka taea te wehewehe i te nucleoside phosphorylase ki te purine nucleoside phosphorylase me te pyrimidine nucleoside phosphorylase e ai ki te hiahia o ngā turanga nucleoside. Ka taea e te purine nucleoside phosphorylase te huri i te adenosine ki te adenine, te inosine ki te hypoxanthine, te guanosine ki te guanine, me te whakaputa i te ribose phosphate i te wā kotahi. Ka taea e te pyrimidine nucleoside phosphorylase te huri i te uridine ki te uracil me te whakaputa i te ribose phosphate.

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Ī-mēra:lchen@syncozymes.com

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Taipitopito Hua

Ngā Tohu Hua

Momo tauhohenga phosphoryalse nucloside:

Ngā Mōhiohio Hua:

Ngā whākōkī	Waehere Hua	Whakatakotoranga
Paura whākōkī	ES-NP-101~ ES-NP-103	he huinga o te 3 Nuclosidephosphoryalse, 50 mg ia 3 tūemi * 50mg / tūemi, tetahi atu rahinga rānei

Te tukanga tātaritanga tauhohenga nucloside phosphoryalse:

Hei tauira, ka taea te whakamahi i te pūnaha tauhohenga e whai ake nei:
1-10 mg/ml Papatūānuku
10 mg/ml Whākōkī
100 mM Pūhui Phosphate (pH7.0)
Whakatōkia ngā tauhohenga ki tētahi rūrū pāmahana pumau (hei tauira, 30°C, 150rpm) mō ngā hāora 12~24. Whakakorikoria ia ranunga kia puta he pūmua, ā, ka taea te whakamahi i te waikura mō te tātaritanga TLC, HPLC, GC rānei.