Nucloside phosphoryalse (NP)

Whakaahuatanga Poto:

Mo te nucleoside phosphoryalse

E 3 nga momo hua NP enzyme (Tau hei ES-NP-101~ ES-NP-103) i hangaia e SyncoZymes.Ko te ES-NP-101 he purine nucleoside phosphorylase, ES-NP-102 me ES-NP-103 he pyrimidine nucleoside phosphorylase.Ka taea e Nucleosidephosphorylase te tihorea nga nucleosides ki nga turanga me te pentose phosphate.Ka taea te wehewehea te nucleoside phosphorylase ki te purine nucleoside phosphorylase me te pyrimidine nucleoside phosphorylase i runga i te hiahia o nga turanga nucleoside.Ka taea e te purine nucleoside phosphorylase te huri i te adenosine ki te adenine, te inosine ki te hypoxanthine, te guanosine ki te guanine, me te whakaputa ribose phosphate i te wa ano.Ka taea e te Pyrimidine nucleoside phosphorylase te whakauru i te uridine ki te uracil me te whakaputa ribose phosphate.

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Taipitopito Hua

Tohu Hua

Momo tauhohenga Nucloside phosphoryalse:

Mōhiohio Hua:

Nga Enzymes	Waehere Hua	Whakatakotoranga
Te paura Enzyme	ES-NP-101~ ES-NP-103	he huinga o 3 Nuclosidephosphoryalse, 50 mg ia 3 tuemi * 50mg / taonga, etahi atu rahinga ranei

Nga tikanga whakamaarama i nga tauhohenga Nucloside phosphoryalse:

Ka taea te whakamahi i te punaha tauhohenga e whai ake nei hei tohutoro:
1-10 mg/ml Raupapa
10 mg/ml Enzyme
100 mM Pūreihana Phosphate (pH7.0)
Whakanuia nga tauhohenga ki te wiriwiri mahana tonu (hei tauira, 30oC, 150rpm) mo te 12~24 haora.Whakanuia ia ranunga ki te whakaheke i te pūmua, ka taea te whakamahi i te supernatant mo te tātari TLC, HPLC, GC ranei.