Transaldolase (TAL)
★ motuhake tïpako High.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohe he tïpako, he wairewa buffer, enzyme, PLP, Mg2+.
➢ Me whakauru te TAL ki roto i te punaha tauhohenga, i muri i te whakatikanga o te pH me te pāmahana ki te ahua o te tauhohenga.
Tauira 1(Te whakahiato o (2S, 3R) -p-methylsulfonyl phenylserine mai i te p-methylsulfonyl benzaldehyde me te threonine)(1):
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. CN109836362B
Tuhia to korero ki konei ka tukuna mai ki a matou