Cytochrome P450 monooxygenase (CYP)

Whakaahuatanga Poto:

Mo CYP

ES-CYPs: He karaehe o nga enzymes redox me te mercaptan-heme hei pokapū kaha ki te whakakorikori i te whakawhiti hāora.Ko ratou he oxygenases mutunga me te hiahia heme ki te whakahohe hāora.

Ko te Cytochrome P450 monooxygenase te whakakorikori i te waiora, te epoxidation me te hydroxylation o te alkyl, te hydroxylation me te oxidation o te haukini, te oxidation o te whanariki, te dealkylation(te hāora, te whanariki, te haukinia), te whakamohoatanga oxidative, te deamination me te dehalogenation.
E 8 nga momo hua cytochrome P450 monooxygenase (Tau hei ES-CYP-101～ES-CYP-108) i hangaia e SyncoZymes.

Pūkoro/Wechat/WhatsApp: +86-13681683526

Ī-Mēra:lchen@syncozymes.com

Tukuna he imeera ki a matou

Taipitopito Hua

Tohu Hua

Mō CYP:

Momo tauhohenga catalytic:

Mōhiohio Hua:

Nga Enzymes	Kete Tirohanga (SynKit)	Whakatakotoranga
Te paura Enzyme	ES-CYP-101~ ES-CYP-108	he huinga o 8 Cytochrome P450 Monooxygenases, 50 mg ia 8 mea * 50mg / mea, etahi atu rahinga ranei
Kete Tirohanga (SynKit)	ES-CYP-800	he huinga o 8 Cytochrome P450 Monooxygenases,1mg ia 8 tuemi * 1mg / mea

Painga:

★ Awhiowhio tïpako whanui.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.

Nga tohutohu mo te whakamahi:

➢ Ko te tikanga, me uru ki roto i te punaha tauhohenga he tïpako, he wairewa buffer (Ko te pH tauhohenga tino pai o te enzyme), te coenzyme (NAD(H) or NADP(H)), te punaha whakahou i te coenzyme (hei tauira, glucose me te glucose dehydrogenase) me te ES-CYP.Ka taea te whakakapi i te punaha whakaahuru me te punaha whakahou ki te hauwai peroxide i roto i te punaha tauhohenga wahanga.
➢ Ko nga momo ES-CYP katoa e pa ana ki nga ahuatanga tauhohenga tino pai me ako takitahi.
➢ Ka taea te aukati i te mahi a te ES-CYP i te papanga kukū teitei.Heoi, ka taea te whakakore i te aukati ma te taapiri puranga o te tïpako.

Tauira Taupānga:

Tauira 1⁽¹⁾:

Tauira 2⁽²⁾:

Tauira 3⁽³⁾:

Tauira 4⁽⁴⁾:

Rokiroki:

Kia 2 tau ki raro -20 ℃.

aro:

Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.

Tohutoro:

1. Zaretzki J, Matlock M, me Swamidass S JJ Chem.Inf.Tauira, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez FA., e tal.J. Am.Chem.Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., me De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallogr.2009, 65, 372.