Whakaahua Matua o te Cytochrome P450 monooxygenase (CYP)

Te monooxygenase cytochrome P450 (CYP)

Whakaahuatanga Poto:

Mō CYP

ES-CYP: He momo whākōkī redox me te mercaptan-heme hei pokapū hohe hei whakatere i te whakawhiti hāora. He oxygenase whakamutunga ēnei, ā, me heme hei whakahohe i te hāora.

Ka whakakorikori te cytochrome P450 monooxygenase i te waikura, te epoxidation me te hydroxylation o te alkyl, te hydroxylation me te waikura o te haukini, te waikura o te whanariki, te dealkylation (hāora, whanariki, haukini), te tango waikura, te deamination me te dehalogenation.
E waru ngā momo hua cytochrome P450 monooxygenase (Tau ko ES-CYP-101～ES-CYP-108) i whakawhanakehia e SyncoZymes.

Waea Pūkoro/Wechat/WhatsApp: +86-13681683526

Ī-mēra:lchen@syncozymes.com

Tukua mai he īmēra ki a mātou

Taipitopito Hua

Ngā Tohu Hua

Mō CYP:

Momo tauhohenga whakakorikori:

Ngā Mōhiohio Hua:

Ngā whākōkī	Kete Tirotiro (SynKit)	Whakatakotoranga
Paura whākōkī	ES-CYP-101~ ES-CYP-108	he huinga o ngā Cytochrome P450 Monooxygenases e 8, 50 mg ia mea 8 ngā tūemi * 50mg ia tūemi, tētahi atu rahinga rānei
Kete Tirotiro (SynKit)	ES-CYP-800	he huinga o ngā Monooxygenases Cytochrome P450 e 8, 1mg ia 8 tūemi * 1mg / tūemi

Ngā Painga:

★ Whānui te whānuitanga o te whāriki.
★ Te tahuritanga teitei.
★ He iti ake ngā hua taha.
★ Ngā āhuatanga tauhohenga ngawari.
★ He pai mō te taiao.

Ngā tohutohu mō te whakamahi:

➢ I te nuinga o te wā, me whakauru te pūnaha tauhohenga i te matūriki, te otinga parepare (te pH tauhohenga tino pai o te whākōkī), te coenzyme (NAD(H) NADP(H) rānei), te pūnaha whakahou coenzyme (hei tauira, te hukahuka me te hukahuka dehydrogenase) me te ES-CYP. Ka taea te whakakapi i te pūnaha whakahou coenzyme me te coenzyme e te hauwai waikura i roto i te pūnaha tauhohenga wāhanga.
➢ Me ako takitahi ngā momo ES-CYP katoa e pā ana ki ngā āhuatanga tauhohenga tino pai.
➢ Ka ārai pea te matūriki, te hua rānei e mau ana i te kukū tiketike i te mahi a te ES-CYP. Heoi, ka taea te whakaiti i te aukati mā te tāpiri puranga o te matūriki.

Ngā Tauira Taupānga:

Tauira 1⁽¹⁾:

Tauira 2⁽²⁾:

Tauira 3⁽³⁾:

Tauira 4⁽⁴⁾:

Rokiroki:

Puritia mō ngā tau e rua i raro i te -20℃.

Whakarongo mai:

Kaua rawa e pā ki ngā āhuatanga tino kino pēnei i: te pāmahana teitei, te pH teitei/iti me te whakarewa waro kukū teitei.

Ngā Tohutoro:

1. Zaretzki J, Matlock M, me Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez FA., e tal. J. Am. Chem. Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., me De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallogr. 2009, 65, 372.