Phenylalanine dehydrogenase (PDH)
Whakaahuatanga Poto:
Mo te Phenylalanine dehydrogenase
ES-PDH: He karaehe o te oxidoreductase.Ka taea e te tauhohenga pai te whakakorikori i te whakakorenga o te tote L phenylalanine i te aroaro o te NAD hei whakaputa i te tote phenylpyruvate, a ka taea e te tauhohenga whakamuri te whakakoi i te whakahiatotanga o te tote waikawa amino rite mai i te tote phenylpyruvate.I whakawhanakehia e Syncozymes nga mea 8 o te phenylalanine dehydrogenase (he nama ES-PDH-101~ES-PDH-108).Ka taea te whakamahi i te ES-PDH ki te whakarite i te tote phenylalanine, te tote waikawa amino rite ranei mai i te tote waikawa phenylpyruvic, i nga hua ranei.
Momo tauhohenga catalytic:
Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-Mēra:lchen@syncozymes.com
| Nga Enzymes | Waehere Hua | Whakatakotoranga |
| Te paura Enzyme | ES-PDH-101~ ES-PDH-108 | he huinga o 8 Nitrile Reductase, 50 mg ia 8 mea * 50mg / mea, etahi atu rahinga ranei |
★ motuhake tïpako teitei.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohe he tïpako, he wairewa buffer, enzyme, coenzyme (NAD(H)), te punaha whakaora i te coenzyme (hei tauira te ammonium formate me te formate dehydrogenase).
➢ Ia momo ES-PDH e rite ana ki nga ahuatanga tauhohenga tino pai, ka taea te ako takitahi mena ka hiahiatia.
➢ Me whakauru te ES-PDH whakamutunga ki te punaha tauhohenga kia mau tonu ai te mahi.
Tauira 1(Ko te koiora o nga päröpü phenylalanine mai i ngä pärönaki phenylpyruvate)(1):
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. Francesca Paradisi, Stuart Collins, et al.Journal of Biotechnology, 2007, 128, 408–411.
