Te whakahekenga o te ene (ERED)
Ka whakakorikorihia e ngā ES-ERED ngā momo papaaho rerekē nā te whānuitanga o ngā papaaho. I te nuinga o te wā, ko te C=C o ngā pūhui α, β-kore mākū me ngā rōpū mimiti-irahiko (tae atu ki te ketone aldehyde, ngā rōpū nitro, ngā waikawa carboxylic, ngā esters, te anhydride, te lactones, te imines, me ētahi atu) ka ngāwari te whakaiti e ngā ES-ERED, engari kāore ngā hononga takirua kāore i whakahohehia.
E 46 ngā momo hua whākōkī ERED (Tau ko ES-ERED-101~ES-ERED-146) i whakawhanakehia e SyncoZymes.
Tikanga whakaterenga:
| Ngā whākōkī | Waehere Hua | Whakatakotoranga |
| Paura whākōkī | ES-ERED-101~ ES-ERED-146 | he huinga o ngā Ene Reductases e 46, 50 mg ia mea 46 ngā tūemi * 50mg ia tūemi, tētahi atu rahinga rānei |
| Kete Tirotiro (SynKit) | ES-ERED-4600 | he huinga o ngā Ene Reductases e 46, 50 mg ia mea 46 ngā tūemi * 50mg ia tūemi, tētahi atu rahinga rānei |
★ Teitei o te motuhaketanga o te papa.
★ He kaha te whiriwhiringa chiral.
★ Te tahuritanga teitei.
★ He iti ake ngā hua taha.
★ Ngā āhuatanga tauhohenga ngawari.
★ He pai mō te taiao.
★ Te haumaru teitei.
➢ I te nuinga o te wā, me whakauru e te pūnaha tauhohenga te matūriki, te otinga parenga (te pH tauhohenga tino pai), ngā coenzymes (NAD(H) NADP(H) rānei), te pūnaha whakaora coenzyme (hei tauira, te glucose me te glucose dehydrogenase) me te ES-ERED.
➢ Ka taea te whakamātautau i ngā ES-ERED katoa i roto i te pūnaha tauhohenga i runga ake nei, mā te Kete Tirotiro ERED (SynKit ERED rānei).
➢ Me ako takitahi ngā momo ES-ERED katoa e pā ana ki ngā āhuatanga tauhohenga tino pai.
➢ Ka ārai pea te matūriki, te hua rānei e mau ana i te kukū tiketike i te mahi a te ES-ERED. Heoi, ka taea te whakaiti i te aukati mā te tāpiri puranga o te matūriki.
Tauira 1 (α,β-unsaturated aldehydes, ketones rānei)(1):
Tauira 2(ngā waikawa carboxylic α,β-unsaturated me ō rātou pānga)(2):
Puritia mō ngā tau e rua i raro i te -20℃.
Kaua rawa e pā ki ngā āhuatanga tino kino pēnei i: te pāmahana teitei, te pH teitei/iti me te whakarewa waro kukū teitei.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, me ētahi atu. .Org.Lett, 2007, 9(26): 5409-5411.
