Nitrile hydratase (NHT)
Whakaahuatanga Poto:
Mō ngā ES-NHT
Ngā ES-NHT: he momo metalloenzyme, e whakamākūkū ana i ngā nitrile ki roto i te amide tuatahi e rite ana. E whakamahia whānuitia ana ēnei ehara i te mea mō te hanga waikawa amino, amide, waikawa carboxylic me ō rātou hua anake, engari mō te whakarite rongoā chiral hoki. E 24 ngā momo nitrile hydratases i whakawhanakehia e SyncoZymes (Tau ko ES-NHT-101～ES-NHT-124).
Momo tauhohenga whakakorikori:
Waea Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-mēra:lchen@syncozymes.com
| Ngā whākōkī | Waehere Hua | Whakatakotoranga |
| Paura whākōkī | ES-NHT-101~ ES-NHT-124 | he huinga o ngā Nitrile Hydratases e 24, 50 mg ia mea 24 ngā tūemi * 50mg ia tūemi, tētahi atu rahinga rānei |
| Kete Tirotiro (SynKit) | ES-NHT-2400 | he huinga o ngā Nnitrile Hydratases e 24, 1 mg ia 24 ngā tūemi * 1mg / tūemi |
★ Teitei o te motuhaketanga o te papa.
★ He kaha te whiriwhiringa chiral.
★ Te tahuritanga teitei.
★ He iti ake ngā hua taha.
★ Ngā āhuatanga tauhohenga ngawari.
★ He pai mō te taiao.
➢ I te nuinga o te wā, me whakauru te pūnaha tauhohenga i te papa, te otinga ārai (te pH tauhohenga tino pai) me te ES-NHT.
➢ Ka taea te whakamātautau i ngā ES-NHT katoa i roto i te pūnaha tauhohenga i runga ake nei, mā te Kete Tirotiro NHT (SynKit NHT rānei).
➢ Me ako takitahi ngā momo ES-NHT katoa e pā ana ki ngā āhuatanga tauhohenga tino pai.
➢ Ka ārai pea te matūriki, te hua rānei e mau ana i te kukū teitei i te mahi a te ES-NHT. Heoi, ka taea te whakaiti i te aukati mā te tāpiri puranga o te matūriki, te hono atu ki ngā amidase, te tango hua rānei.
Tauira 1 (te hononga ki te amidase)(1):
Tauira 2(2):
Puritia mō ngā tau e rua i raro i te -20℃.
Kaua rawa e pā ki ngā āhuatanga tino kino pēnei i: te pāmahana teitei, te pH teitei/iti me te whakarewa waro kukū teitei.
1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, me ētahi atu. Tetrahedron lett, 2010, 51: 1639-1641.
