Nitrile reductase (NRED)
Whakaahuatanga Poto:
Mo te Nitrile reductase
E toru nga momo hua enzyme NRED (Tau hei ES-NRED101~ ES-NRED103) i hangaia e SyncoZymes.Ka taea e NRED te whakaheke i te roopu nitrile ki te amine.I roto i te tukanga o te catalysis, ka hiahiatia te NADPH hei kawe hauwai.
Momo tauhohenga reductasecatalytic Nitrile
Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-Mēra:lchen@syncozymes.com
| Nga Enzymes | Waehere Hua | Whakatakotoranga |
| Whakatakotoranga | ES- NRED -101~ ES- NRED -103 | he huinga o 3 Nitrile Reductase, 50 mg ia 3 tuemi * 50mg / mea, etahi atu rahinga ranei |
Ka taea te whakamahi i te punaha tauhohenga e whai ake nei hei tohutoro:
1-10 mg/ml Raupapa
10 mg/ml Enzyme
10% (v/v) Whakarewa pararopi (1)
100 mM Pūreihana Phosphate (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Te hukahuka
2 mg/ml Glucose dehydrogenase (GDH)
Whakanuia nga tauhohenga ki te wiriwiri mahana tonu (hei tauira, 30oC, 150rpm) mo te 24~48 haora.Whakanuia ia ranunga ki te whakaheke i te pūmua, ka taea te whakamahi i te supernatant mo te TLC, HPLC, GC ranei tātaritanga.
Tuhipoka (1): Ko te whakarewa pararopi (penei i te DMSO, methanol, acetonitrile, IPA, me etahi atu) he mea whiriwhiri, whakamahia mo nga taputapu korekore.
