Transaminase (ATA)
Ngā whākōkī: He whakakorikori koiora macromolecular, ko te nuinga o ngā whākōkī he pūmua.
Ngā Transaminases: He momo whākōkī e whakateretere ana i te whakawhiti amino i waenga i ngā waikawa amino me ngā waikawa keto. Ko ngā Transaminases ngā whākōkī koiora matua i roto i te hanga kore taurite me te whakataunga racemic o ngā amine chiral.
E whā ngā momo o te aminotransferase, e ai ki te raupapatanga me te hanganga: Ⅰ, Ⅱ, Ⅲ me Ⅳ. Nō roto ngā ω-aminotransferase i ngā transaminases o te momo II, ā, e whakamahia whānuitia ana i roto i te hanga i ngā amine chiral me ngā waikawa amino taiao kore, pērā i ngā waikawa amino β-.
ω-aminotransferases: I te nuinga o te wā, ko te ω-transaminase e pā ana ki tētahi momo whākōkī e whakawhiti ana i te haukini me te kore he waikawa α-amino hei matū, hei hua rānei.
Tikanga whakaterenga:
| Ngā whākōkī | Waehere Hua | Waehere Hua |
| Paura whākōkī | ES-ATA-101~ ES-ATA-165 | he huinga o te 65 ω-Transaminases, 50 mg ia 65 ngā tūemi * 50mg/ tūemi, tētahi atu rahinga rānei |
| Kete Tirotiro (SynKit) | ES-ATA-6500 | he huinga o te 65 ω-Transaminases, 1 mg ia 65 tūemi * 1mg / tūemi |
★ Teitei o te motuhaketanga o te papa.
★ He kaha te whiriwhiringa chiral.
★ Te tino whai huatanga o te tahuritanga.
★ He iti ake ngā hua taha.
★ Ngā āhuatanga tauhohenga ngawari.
★ He pai mō te taiao.
➢ Me whakahaere te tātari whākōkī mō ngā matū motuhake nā te mea he motuhake te matū, ā, me tiki he whākōkī e whakaoho ana i te matū ūnga me te pānga whakaoho pai rawa atu.
➢ Kaua rawa e pā ki ngā āhuatanga tino kino pēnei i te: pāmahana teitei, pH teitei/iti me te whakarewa waro me te kukū teitei.
➢ I te nuinga o te wā, me whakauru ki te pūnaha tauhohenga te matūriki, te otinga ārai, te kaituku amino (pēnei i ngā waikawa amino me te 1-phenyl ethylamine) te kaiwhiwhi rānei (pēnei i ngā waikawa keto), te coenzyme (PLP), te cosolvent (pēnei i te DMSO).
➢ Me tāpiri te ATA ki te pūnaha tauhohenga i muri i te whakarerekētanga o te pH me te pāmahana ki te āhua o te tauhohenga.
➢ He rerekē ngā āhuatanga tauhohenga tino pai o ngā momo ATA katoa, nō reira me ako takitahi ia āhuatanga.
Tauira 1 (te hanga o Sitagliptin, te hanga kore taurite)(1):
Tauira 2 (Mexiletine, Huinga o te taumira nekeneke me te hanganga kore taurite)(2):
1 Savile CK, Janey JM, Mundorff EC, me ētahi atu. Pūtaiao, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Reta pararopi, 2009,11(21):4810-4812.
