Phenylalanine ammonia lyase (PAL)
Nga Enzymes | Waehere Hua | Whakatakotoranga |
Te paura Enzyme | ES-PAL-101~ ES-PAL-110 | he huinga o te 10 L-phenylalanine ammonia-lyase, 50 mg ia 10 mea * 50mg / mea, etahi atu rahinga ranei |
★ motuhake tïpako teitei.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me uru ki roto i te punaha tauhohenga te tïpako, te wairewa buffer, me te enzyme.
➢ Ia momo ES-PAL e rite ana ki nga ahuatanga tauhohenga tino pai, ka taea te ako takitahi.
➢ Ko te ES-PAL me whakauru whakamutunga ki te punaha tauhohenga kia mau tonu te mahi.
Tauira 1(Ko te koiora o te waikawa cinnamic whakawhiti mai i te phenylalanine)(1):
Tauira 2(Ko te koiora o te päröpü phenylalanine mai i te pärönaki waikawa trans-cinnamic)(2):
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. Zachary Mays, Karishma Mohan,et al.Chem Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,et al. Tetrahedron,2016. 72, 7343-7347.