Phenylalanine dehydrogenase (PDH)
Whakaahuatanga Poto:
Mō te Phenylalanine dehydrogenase
ES-PDH: He momo oxidoreductase. Ka taea e te tauhohenga pai te whakakorikori i te whakakorenga waikura o te tote phenylalanine L i te aroaro o te NAD hei whakaputa i te tote phenylpyruvate, ā, ka taea e te tauhohenga whakamuri te whakakorikori i te hanganga o te tote waikawa amino e rite ana mai i te tote phenylpyruvate. I whakawhanakehia e Syncozymes e 8 ngā tūemi o te phenylalanine dehydrogenase (kua tau ES-PDH-101~ES-PDH-108). Ka taea te whakamahi i te ES-PDH hei whakarite i te tote phenylalanine, i te tote waikawa amino rānei e rite ana mai i te tote waikawa phenylpyruvic, i ngā mea rānei i ahu mai i ngā matū.
Momo tauhohenga whakakorikori:
Waea Pūkoro/Wechat/WhatsApp: +86-13681683526
Ī-mēra:lchen@syncozymes.com
| Ngā whākōkī | Waehere Hua | Whakatakotoranga |
| Paura whākōkī | ES-PDH-101~ ES-PDH-108 | he huinga o te 8 Nitrile Reductase, 50 mg ia 8 ngā tūemi * 50mg ia tūemi, tētahi atu rahinga rānei |
★ Teitei o te motuhaketanga o te papa.
★ He kaha te whiriwhiringa chiral.
★ Te tahuritanga teitei.
★ He iti ake ngā hua taha.
★ Ngā āhuatanga tauhohenga ngawari.
★ He pai mō te taiao.
➢ I te nuinga o te wā, me whakauru te pūnaha tauhohenga i te matūriki, te otinga parepare, te whākōkī, te koehaukini (NAD(H)), te pūnaha whakaora koehaukini (hei tauira, te ammonium formate me te formate dehydrogenase).
➢ Ia momo ES-PDH e rite ana ki ngā āhuatanga tauhohenga tino pai, ka taea te ako takitahi mēnā e tika ana.
➢ Me tāpiri te ES-PDH ki te pūnaha tauhohenga hei whakamutunga hei pupuri i te mahi.
Tauira 1 (Te hanga koiora o ngā matū phenylalanine mai i ngā matū phenylpyruvate)(1):
Puritia mō ngā tau e rua i raro i te -20℃.
Kaua rawa e pā ki ngā āhuatanga tino kino pēnei i: te pāmahana teitei, te pH teitei/iti me te whakarewa waro kukū teitei.
1. Francesca Paradisi, Stuart Collins, me ētahi atu. Journal of Biotechnology, 2007, 128, 408–411.
