Phosphokinase (PKase)
Nga Enzymes | Waehere Hua | Whakatakotoranga |
Kete Tirohanga (SynKit) | ES-PKase-101~ES-PKase-121 | he huinga o 21 Ketoreductases, 1 mg ia 21 tūemi * 1mg / tūemi |
★ motuhake tïpako High.
★ He kaha te whiriwhiringa o te chiral.
★ Te hurihanga teitei.
★ He iti ake nga hua.
★ Nga tikanga tauhohenga ngawari.
★ He pai te taiao.
➢ Ko te tikanga, me whakauru te punaha tauhohenga he tïpako, he wairewa buffer, enzyme, ATP, Mg2+.
➢ Me whakauru te PKase whakamutunga ki roto i te punaha tauhohenga, i muri i te whakatikanga o te pH me te pāmahana ki te ahuatanga tauhohenga.
Tauira 1(Te whakahiato o te ribose phosphate nicotinamide mai i te ribose nicotinamide)(1):
Tuhipoka: Ko nga tauira tono me nga tohutoro e tohu ana i te whānuitanga tono o PKase mo te maamaatanga o te maarama, me te kore e rite ki te whaariki motuhake o SyncoZymes.
Kia 2 tau ki raro -20 ℃.
Kaua rawa e pa atu ki nga ahuatanga tino kino penei: te pāmahana teitei, te pH teitei/iti me te whakarewa pararopi kukū teitei.
1. Khan, Javed A., Song Xiang, and Liang Tong.Hanganga 15.8 (2007): 1005-1013.